ABSTRACT
To investigate the secondary metabolites of endophytic fungi Pericinia sp. F-31. Column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds. Two compounds were isolated from the fermentation broth of Periconia sp. Their structures were identified as 5-(1-hydroxyhexyl) -6-methyl-2H-pyran-2-one (1) and 2-(3-hydroxy-4-methylphenyl) -propanoic acid (2). Compound 1 was a new lactone compound, compound 2 was new natural product, and the NMR data of compound 2 was reported for the first time.
Subject(s)
Annona , Microbiology , Ascomycota , Chemistry , Genetics , Metabolism , Drugs, Chinese Herbal , Chemistry , Metabolism , Endophytes , Chemistry , Genetics , Metabolism , Lactones , Chemistry , Metabolism , Mass Spectrometry , Molecular StructureABSTRACT
The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on physico-chemical properties and spectroscopic data, their structures were identified as isobavachalcone (1), genistein (2), norartocarpetin (3), albanin A (4), guangsangon E (5), mulberrofuran F (6), chalcomoracin (7), kuwanon J (8). Compounds 3-6 were isolated from the cell cultures of M. alba for the first time.
Subject(s)
Benzofurans , Cell Culture Techniques , Methods , Chalcones , Chromatography, Gel , Methods , Chromatography, High Pressure Liquid , Dextrans , Genistein , Morus , Chemistry , Cell Biology , Plant Leaves , Chemistry , Cell Biology , Plants, Medicinal , Chemistry , Cell Biology , Silica Gel , TerpenesABSTRACT
Objective: To study the chemical constituents from the cane of Pueraria lobata. Methods: The compounds were isolated by column chromatography over silica gel and purified by Sephadex LH-20 column chromatography and preparative TLC. The structures were elucidated by means of physicochemical properties and spectroscopic analyses. Results: Eleven compounds were separated and identified as: dehydrovomifoliol (1), blumenol A (2), 3β-hydroxy-5α,6α- epoxy-7-megastigmen-9-one (3), liquiritigenin (4), garbanzol (5), ferulaldehyde (6), 2,4-dihydroxybenzaldehyde (7), 4-hydroxy-2-methoxybenzaldehyde (8), (-)-tuberosin (9), coumestrol (10), and β-sitosterol (11). Conclusion: Compounds 1-3 are sesquiterpenoids, which are firstly isolated from the plants of Pueraria DC., and compounds 4-8 are obtained from the plants of Pueraria DC. for the first time.